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Registro Completo |
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Biblioteca(s): |
Biblioteca Rui Tendinha. |
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Data corrente: |
25/09/2014 |
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Data da última atualização: |
01/09/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ABREU, P. M. V.; GASPAR, C. G.; BUSS, D. S.; VENTURA, J. A.; FERREIRA, P. C. G.; FERNANDES, P. M. B. |
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Afiliação: |
Paolla M. V. Abreu, Universidade Federal do Espíríto Santo; Clicia G. Gaspar, Univervidade Federal do Rio de Janeiro; David S. Buss, Universidade Federal do Espírito Santo; Jose Aires Ventura, Incaper; Paulo C. G. Ferreira, Universidade Federal do Rio de Janeiro; Patricia M. B. Fernandes, Universidade Federal do Espírito Santo. |
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Título: |
Carica papaya MicroRNAs Are Responsive to Papaya meleira virus Infection. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
PLOS ONE, v. 9, issue 7, p. 1-13, july 2014. |
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Páginas: |
13 p. |
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Descrição Física: |
il., color. |
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ISSN: |
1932-6203 |
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DOI: |
10.1371 |
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Idioma: |
Português |
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Conteúdo: |
MicroRNAs are implicated in the response to biotic stresses. Papaya meleira virus (PMeV) is the causal agent of sticky disease, a commercially important pathology in papaya for which there are currently no resistant varieties. PMeV has a number of unusual features, such as residence in the laticifers of infected plants, and the response of the papaya to PMeV infection is not well understood. The protein levels of 20S proteasome subunits increase during PMeV infection, suggesting that proteolysis could be an important aspect of the plant defense response mechanism. To date, 10,598 plant microRNAs have been identified in the Plant miRNAs Database, but only two, miR162 and miR403, are from papaya. In this study, known plant microRNA sequences were used to search for potential microRNAs in the papaya genome. A total of 462 microRNAs, representing 72 microRNA families, were identified. The expression of 11 microRNAs, whose targets are involved in 20S and 26S proteasomal degradation and in other stress response pathways, was compared by real-time PCR in healthy and infected papaya leaf tissue. We found that the expression of miRNAs involved in proteasomal degradation increased in response to very low levels of PMeV titre and decreased as the viral titre increased. In contrast, miRNAs implicated in the plant response to biotic stress decreased their expression at very low level of PMeV and increased at high PMeV levels. Corroborating with this results, analysed target genes for this miRNAs had their expression modulated in a dependent manner. This study represents a comprehensive identification of conserved miRNAs inpapaya. The data presented here might help to complement the available molecular and genomic tools for the study of papaya. The differential expression of some miRNAs and identifying their target genes will be helpful for understanding the regulation and interaction of PMeV and papaya. MenosMicroRNAs are implicated in the response to biotic stresses. Papaya meleira virus (PMeV) is the causal agent of sticky disease, a commercially important pathology in papaya for which there are currently no resistant varieties. PMeV has a number of unusual features, such as residence in the laticifers of infected plants, and the response of the papaya to PMeV infection is not well understood. The protein levels of 20S proteasome subunits increase during PMeV infection, suggesting that proteolysis could be an important aspect of the plant defense response mechanism. To date, 10,598 plant microRNAs have been identified in the Plant miRNAs Database, but only two, miR162 and miR403, are from papaya. In this study, known plant microRNA sequences were used to search for potential microRNAs in the papaya genome. A total of 462 microRNAs, representing 72 microRNA families, were identified. The expression of 11 microRNAs, whose targets are involved in 20S and 26S proteasomal degradation and in other stress response pathways, was compared by real-time PCR in healthy and infected papaya leaf tissue. We found that the expression of miRNAs involved in proteasomal degradation increased in response to very low levels of PMeV titre and decreased as the viral titre increased. In contrast, miRNAs implicated in the plant response to biotic stress decreased their expression at very low level of PMeV and increased at high PMeV levels. Corroborating with this results, analysed target genes for thi... Mostrar Tudo |
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Palavras-Chave: |
Carica papaya; Doenças; Mamão; Meleira; MiRNAs; PMeV; Vírus. |
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Thesaurus NAL: |
Carica papaya; Disease; Meleira; MiRNAs; PMeV; Virus. |
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Categoria do assunto: |
-- |
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URL: |
http://biblioteca.incaper.es.gov.br/digital/bitstream/item/397/1/Carica-papaya-microRNAS-Are-responsive-to-papaya.pdf
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Marc: |
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245 $aCarica papaya MicroRNAs Are Responsive to Papaya meleira virus Infection.$h[electronic resource]
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520 $aMicroRNAs are implicated in the response to biotic stresses. Papaya meleira virus (PMeV) is the causal agent of sticky disease, a commercially important pathology in papaya for which there are currently no resistant varieties. PMeV has a number of unusual features, such as residence in the laticifers of infected plants, and the response of the papaya to PMeV infection is not well understood. The protein levels of 20S proteasome subunits increase during PMeV infection, suggesting that proteolysis could be an important aspect of the plant defense response mechanism. To date, 10,598 plant microRNAs have been identified in the Plant miRNAs Database, but only two, miR162 and miR403, are from papaya. In this study, known plant microRNA sequences were used to search for potential microRNAs in the papaya genome. A total of 462 microRNAs, representing 72 microRNA families, were identified. The expression of 11 microRNAs, whose targets are involved in 20S and 26S proteasomal degradation and in other stress response pathways, was compared by real-time PCR in healthy and infected papaya leaf tissue. We found that the expression of miRNAs involved in proteasomal degradation increased in response to very low levels of PMeV titre and decreased as the viral titre increased. In contrast, miRNAs implicated in the plant response to biotic stress decreased their expression at very low level of PMeV and increased at high PMeV levels. Corroborating with this results, analysed target genes for this miRNAs had their expression modulated in a dependent manner. This study represents a comprehensive identification of conserved miRNAs inpapaya. The data presented here might help to complement the available molecular and genomic tools for the study of papaya. The differential expression of some miRNAs and identifying their target genes will be helpful for understanding the regulation and interaction of PMeV and papaya.
650 $aCarica papaya
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650 $aPMeV
650 $aVirus
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773 $tPLOS ONE$gv. 9, issue 7, p. 1-13, july 2014.
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Registro original: |
Biblioteca Rui Tendinha (BRT) |
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Biblioteca |
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Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
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| Registros recuperados : 5 | |
| 3. |  | ABREU, P. M. V.; GASPAR, C. G.; BUSS, D. S.; VENTURA, J. A.; FERREIRA, P. C. G.; FERNANDES, P. M. B. Carica papaya MicroRNAs Are Responsive to Papaya meleira virus Infection. PLOS ONE, v. 9, issue 7, p. 1-13, july 2014. 13 p. il., color.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Biblioteca Rui Tendinha. |
|    |
| 4. | | DE FARIAS VIÉGAS AGUIJE, G. M.; ZORZAL, P. B.; BUSS, D. S.; VENTURA, J. A.; FERNANDES, P. M. B.; FERNANDES, A. A. R. Cell wall alterations in the leaves of fusariosis-resistant and susceptible pineapple cultivars. Plant Cell Reports, v. 29, n. 10, p. 1109-1117, 2010.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Biblioteca Rui Tendinha. |
|  |
| 5. | | KORRES, A. M. N.; AQUIJE, G. M. de F. V.; BUSS, D. S.; VENTURA, J. A.; FERNANDES, P. M. B.; FERNANDES, A. A. R. Comparison of biofilm and attachment mechanisms of a Phytopathological and Clinical Isolate of Klebsiella pneumoniae Subsp. pneumoniae. The Scientific World Journal, vol. 2013, Article ID 925375, 6p., 2013. 6p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Biblioteca Rui Tendinha. |
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| Registros recuperados : 5 | |
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| Expressão de busca inválida. Verifique!!! |
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